ArticleApplications in plant sciences
A cost-effective DNA extraction protocol for long-read sequencing in non-model plants.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- A cost-effective DNA extraction protocol for long-read sequencing in non-model plants.Applications in plant sciencesArticle
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Premise: Third-generation sequencing has revolutionized genomics, enabling in-depth analysis of genome sequence, structure, and epigenetic features. Yet, extracting high-quality DNA for long-read sequencing remains a bottleneck-particularly in non-model plants, such as mature trees growing in natural environments, which often contain abundant endogenous compounds that hinder extraction and downstream applications. Methods and Results: We developed an optimized, robust, and cost-effective DNA extraction protocol that yields high-quality DNA suitable for Oxford Nanopore Technologies and PacBio sequencing. Validation across diverse taxa-including six Conclusions: Compared to commercial kits and standard protocols, this approach achieved superior DNA integrity and yield without specialized equipment, offering an accessible solution for researchers working with challenging plant species.
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Registered trials
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