Development and evaluation of a real-time PCR assay for the detection of bovine Milk in foods.

Sarah R Stadig, Anne C Eischeid

Abstract read

In one paragraph

Article in Food chemistry. Molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Sarah R StadigU.S. Food and Drug Administration, Human Foods Program, Office of Laboratory Operations and Applied Science, Office of Chemistry and Toxicology, 5001 Campus Drive, College Park, MD 20740, USA.

Anne C EischeidU.S. Food and Drug Administration, Human Foods Program, Office of Laboratory Operations and Applied Science, Office of Chemistry and Toxicology, 5001 Campus Drive, College Park, MD 20740, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Food Drug & Cosmetic Act designates milk as a major food allergen and requires its labeling on food packages. As such, robust, sensitive detection methods are required to ensure accurate labeling. Immunoassays such as ELISAs detect the allergenic proteins but may be limited by cross-reactivity and protein degradation that occurs during food processing. DNA-based detection methods are often less affected by food processing and offer a robust alternative. In this work, a real-time PCR assay targeting the COI mitochondrial gene for

Indexed as

AllergenBovine milk -COIChocolateMitochondrialReal-time PCR

Identifiers

PMID42631194

DOI10.1016/j.fochms.2026.100447

PMCPMC13495698

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.