ArticleiScience2026
Reconstruction of xenogeneic seminiferous tubule-like structures in mouse testis using cryopreserved rat testicular cells.
Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 authors.
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Abstract
Spermatogonial stem cells sustain spermatogenesis within a Sertoli cell-organized niche, yet xenogeneic spermatogenic differentiation after transplantation into host mouse seminiferous tubules has been reported in only a limited number of donor species. Here, we tested whether host niche engineering could support xenogeneic spermatogenic differentiation. Immunodeficient mouse testes were preconditioned by busulfan-mediated germ cell depletion followed by intratubular benzalkonium chloride treatment to reduce Sertoli cells, and cryopreserved rat testicular cells were transplanted into the modified tubules. Donor-derived germ cells and Sertoli cells colonized the basement membrane, reconstructed seminiferous tubule-like structures, and supported advanced spermatogenic differentiation, including elongating spermatids. These findings show that frozen-thawed donor testicular cells can organize seminiferous tubule-like structures across species boundaries and provide a framework for investigating engineered testicular niches.
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