Evidence map›Paper›PMID 42627547›Full record

ArticleMolecular biology reports2026

Icaritin improves pregnancy outcomes in endometriosis by inhibiting ovarian granulosa cell senescence.

Yalun He, Jie Ding, Yangshuo Li, Ji Sun, Cuiping Wei, Na Sun, Runnan Jiang, Ziming Zhao, Xiaolan Liang, Yiming Chen and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yalun He *Gynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China.
Jie Ding *Gynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China.
Yangshuo Li *Gynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China.
Ji SunRehabilitation Department of Integrated Traditional Chinese and Western Medicine, Beidaihe Rest and Recuperation Center, Qinhuangdao, 066000, Hebei, China.
Cuiping WeiRehabilitation Department of Integrated Traditional Chinese and Western Medicine, Beidaihe Rest and Recuperation Center, Qinhuangdao, 066000, Hebei, China.
Na SunRehabilitation Department of Integrated Traditional Chinese and Western Medicine, Beidaihe Rest and Recuperation Center, Qinhuangdao, 066000, Hebei, China.
Runnan JiangRehabilitation Department of Integrated Traditional Chinese and Western Medicine, Beidaihe Rest and Recuperation Center, Qinhuangdao, 066000, Hebei, China.
Ziming ZhaoRehabilitation Department of Integrated Traditional Chinese and Western Medicine, Beidaihe Rest and Recuperation Center, Qinhuangdao, 066000, Hebei, China.
Xiaolan LiangGynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China.
Yiming ChenGynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China.
Wen ChengGynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China.
Litai ZhangRehabilitation Department of Integrated Traditional Chinese and Western Medicine, Beidaihe Rest and Recuperation Center, Qinhuangdao, 066000, Hebei, China.
Di SongDepartment of Assisted Reproduction, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China. songdi1983@126.com.
Shuai SunGynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China. susu_n115@163.com.
Chaoqin YuGynecology Department of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China. cqyu@smmu.edu.cn.

Funding

Medical innovation research Project of Science and Technology Innovation Action Plan of Shanghai Municipal Science and Technology Commission 20Z21900405National Natural Science Foundation of China 82074206Shanghai Renowned Veteran TCM Practitioners' Academic and Clinical Experience Research Studio SHGZS-202240
6 · The paper itself

Abstract

backgroundEndometriosis (EMS) is an oestrogen-dependent chronic inflammatory disease that primarily manifests as chronic pelvic pain and infertility. EMS-associated infertility is closely linked to diminished ovarian function and oocyte quality, with the aberrant function of granulosa cells (GCs). Cellular senescence has a nonnegligible effect on female fertility. GCs in EMS patients may undergo senescence, subsequently compromising their nutritional support to oocytes and impairing ovarian function, ultimately contributing to infertility. Icaritin (Ica) possesses various biological activities including anti-tumour, anti-inflammatory, anti-oxidation and anti-aging.

methodsFollicular fluid (FF) was collected from normal control females and EMS patients. GCs were extracted from FF for RNA sequencing and bioinformatic analysis. NADH: ubiquinone oxidoreductase core subunit S8 (NDUFS8)-knockdown KGN cells were established using shRNA-mediated lentiviral transduction. Pro-oxidants and oxidation scavengers were employed to modulate reactive oxygen species (ROS) levels. Network pharmacology was employed to analyse potential mechanisms of Ica in treating EMS. Ica was employed to intervene EMS-FF-treated KGN cells and EMS mice.

resultsIca significantly reduced degrees of peritoneal adhesions, decreased quantities and volumes of ectopic lesions and improved reproductive capacity in EMS mice. Moreover, Ica may inhibit ovarian GC senescence and improve ovarian function in EMS mice by regulating NDUFS8/ROS/P53/P21 axis. In vitro experiments demonstrated that Ica may inhibit EMS-FF-induced KGN cell senescence by regulating NDUFS8/ROS/P53/P21 axis.

conclusionsThese findings identify Ica as a GC senescence-inhibiting agent with therapeutic potential in EMS-associated infertility.

Indexed as

Cellular SenescenceEndometriosisFlavonoidsGranulosa CellsAdultAnimalsFemaleFollicular FluidHumansInfertility, FemaleMicePregnancyPregnancy OutcomeReactive Oxygen SpeciesFlavonoidsicaritinReactive Oxygen SpeciesCellular senescenceEndometriosisGranulosa cellIcaritinInfertilityNDUFS8

Identifiers

PMID42627547
PMCPMC13498657

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.