ArticleChemistry & biodiversity2026
Unveiling the Bioactive Potential of Shilajit: Integrated Chemical Profiling and Antimicrobial Evaluation via Chromatographic, Spectroscopic, and In Silico Approaches.
Article in Chemistry & biodiversity, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Shilajit is a herbomineral exudate widely used in traditional medicine and composed mainly of humic substances and low-molecular-weight organic constituents. However, the chemical composition and biological relevance of its low-molecular-weight fraction remain poorly understood. In this study, sequential fractionation was employed to obtain humins (HM), humic acid (HA), fulvic acid (FA), and a low-molecular-weight residual fraction (RF). FTIR analysis supported successful fractionation, with HA and FA exhibiting characteristic humic-like functional groups, while the RF showed reduced polymeric features consistent with enrichment in smaller organic molecules. Comprehensive chemical profiling using multi-solvent GC-MS following derivatization enabled the detection of structurally diverse metabolites. The RF exhibited a broader metabolite profile than whole Shilajit, with 36 low-molecular-weight compounds tentatively identified, including phenolics, aromatic acids, esters, flavanol derivatives, and sterol-related compounds. SEM-EDS analysis revealed heterogeneous morphology and elemental composition. In silico ADMET predictions suggested favorable gastrointestinal absorption and low predicted toxicity for most compounds. Antimicrobial assays demonstrated stronger antibacterial activity of the RF compared to whole Shilajit, while antifungal activity remained limited. Overall, the findings highlight the biological significance of low-molecular-weight constituents in Shilajit.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.