ArticleIranian journal of microbiology2026
Isolation and characterization of bacteriophages against antibiotic-resistance
Article in Iranian journal of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background and Objectives: As an important opportunistic pathogen, Materials and Methods: Fifty clinical isolates of Results: A considerable proportion of the clinical isolates showed multidrug-resistant phenotypes, and a considerable number were also classified as extensively drug-resistant. Four lytic bacteriophages, designated HSP1-HSP4, were successfully isolated and purified. All produced clear plaques and reached high titers. Host range analysis showed variable lytic spectra, with HSP1 exhibiting the broadest activity and showing lytic activity against 70% of the tested isolates, followed by HSP2 (58%), HSP3 (48%), and HSP4 (40%). Adsorption analysis indicated that over 80% of phage particles attached to their host cells within 10 minutes, while HSP1 exhibited the highest adsorption efficiency at 93.6%. A short latent period of approximately 20 minutes was observed for all four phages, and burst sizes ranged from 56 to 65 PFU/cell. Transmission electron microscopy revealed isometric heads and contractile tails, morphologically consistent with tailed phages of the class Conclusion: Hospital wastewater can serve as a practical source for the isolation of lytic bacteriophages active against antibiotic-resistant
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