ArticlePhysiologia plantarum
Biocontrol of Vitis vinifera Gray Mold via a Culture Filtrate of Trichoderma afroharzianum.
Article in Physiologia plantarum. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Botrytis cinerea is a devastating pathogen in viticulture, causing gray mold, and bunch rot. Under 25°C, 90% relative humidity and 36 h wetness duration, B. cinerea can lead to yield losses ranging from 20% to 50% in grapevines. This study evaluated the antifungal effects of Trichoderma afroharzianum secondary metabolite filtrates (TSMF) against the B. cinerea isolate MBc5. In vitro dual culture assays revealed that 10 T. afroharzianum isolates inhibited the colony growth of B. cinerea by 42%-88%. TSMF were collected after 30 days of incubation in yeast peptone glucose (YPG) medium and incorporated into potato dextrose agar (PDA) at 10% (v/v) for inhibition testing against B. cinerea. The filtrates significantly inhibited B. cinerea colony growth by 72%-92%. Based on these results, three highly effective isolates (Tr28, Tr138, and Tr153) were selected for in vivo assays. Leaves and bunches were inoculated with B. cinerea (1 × 10
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