Evidence map›Paper›PMID 42624105›Full record

ArticleStructure (London, England : 1993)2026

Structural determinants of endopilus assembly, stability, and functional specificity in bacterial type II secretion.

Maylis Lejeune, Stefaniia Ivashchenko, Régine Dazzoni, Benjamin Bardiaux, Ravi R Sonani, Matthijn Vos, Theis Jacobsen, Edward H Egelman, Michael Nilges, Olivera Francetic and 2 more

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Article in Structure (London, England : 1993), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Maylis LejeuneInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Bacterial Transmembrane Systems Unit, 75015 Paris, France.
Stefaniia IvashchenkoInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Structural Bioinformatics Unit, 75015 Paris, France.
Régine DazzoniInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Bacterial Transmembrane Systems Unit, 75015 Paris, France.
Benjamin BardiauxInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Bacterial Transmembrane Systems Unit, 75015 Paris, France.
Ravi R SonaniDepartment of Biochemistry and Molecular Genetics, University of Virginia Medical School, Charlottesville, VA, USA.
Matthijn VosInstitut Pasteur, Université Paris Cité, Nanoimaging Core Facility, 75015 Paris, France.
Theis JacobsenInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Structural Bioinformatics Unit, 75015 Paris, France.
Edward H EgelmanDepartment of Biochemistry and Molecular Genetics, University of Virginia Medical School, Charlottesville, VA, USA.
Michael NilgesInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Structural Bioinformatics Unit, 75015 Paris, France.
Olivera FranceticInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Biochemistry of Macromolecular Interactions Unit, 75015 Paris, France. Electronic address: olivera.francetic@pasteur.fr.
Vladimir E ShevchikUniversité Lyon 1, INSA Lyon, CNRS UMR 5240 Microbiologie Adaptation et Pathogénie, Villeurbanne, France. Electronic address: vladimir.shevchik@insa-lyon.fr.
Nadia Izadi-PruneyreInstitut Pasteur, Université Paris Cité, CNRS UMR3528, Bacterial Transmembrane Systems Unit, 75015 Paris, France. Electronic address: nadia.izadi@pasteur.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gram-negative bacteria employ the type II secretion system (T2SS) to transport folded protein effectors via a periplasmic helical polymer called the endopilus, composed of one major and four minor pilin subunits. Endopili resemble type IV pili but feature a conserved calcium-binding site stabilizing their major pilins. Endopilus polymerization is coupled to substrate translocation through a dedicated outer membrane channel. We compared T2SSs from plant and human pathogens, Dickeya dadantii and Klebsiella oxytoca, respectively. Despite different ecological niches and secreted effectors, their major pilins (OutG and PulG) share >77% sequence identity. Using NMR and cryo-EM, we solved structures of calcium-bound OutG monomer, as well as OutG and PulG endopili at 3.6 Å resolution. Combining structural, mutational, and biophysical analyses with in vivo assays, we identified key determinants of secretion specificity and endopilus stability. Our findings reveal how minor sequence variations in conserved nanomachines drive functional adaptation to diverse environments.

Indexed as

Bacterial ProteinsDickeyaFimbriae, BacterialKlebsiella oxytocaType II Secretion SystemsAmino Acid SequenceBinding SitesCalciumCryoelectron MicroscopyModels, MolecularProtein BindingBacterial ProteinsCalciumType II Secretion Systemsbacterial secretion systemcalciumcryo-EMNMRpilus structureprotein stabilitysecretion specificitytype II secretion system

Identifiers

PMID42624105

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.