Evidence map›Paper›PMID 42624100›Full record

ArticleStem cell reports2026

Short-term gonadal cultures are sufficient for germline transmission in a songbird.

Matthew Biegler, Elijah Harter, Asha V Sidhu, Christina Szialta, Gillian Durham, Lotem Tchernichovski, Paul Collier, Ji-Dung Luo, Wei Wang, Ryan MacIsaac and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Matthew BieglerLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA. Electronic address: mbiegler@rockefeller.edu.
Elijah HarterLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA.
Asha V SidhuLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA.
Christina SzialtaLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA.
Gillian DurhamLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA.
Lotem TchernichovskiLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA.
Paul CollierWeill Cornell School of Medicine, New York, NY, USA.
Ji-Dung LuoBioinformatics Resource Center, The Rockefeller University, New York, NY, USA.
Wei WangBioinformatics Resource Center, The Rockefeller University, New York, NY, USA.
Ryan MacIsaacLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA.
Kirubel BelayLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA.
Thomas CarrollBioinformatics Resource Center, The Rockefeller University, New York, NY, USA.
Anna L KeyteLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA; Colossal Biosciences, Dallas, TX, USA. Electronic address: anna@colossal.com.
Erich D JarvisLaboratory of Neurogenetics of Language, The Rockefeller University, New York, NY, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA. Electronic address: ejarvis@rockefeller.edu.

Funding

Genomic Conflict Resolution: Establishment of a new model for unconstrained germline selectionK99HG014014 · NHGRI · ROCKEFELLER UNIVERSITY · PI Matthew Theodor Biegler · 2025 to 2026
$317k
NHGRI NIH HHS K99 HG014014
6 · The paper itself

Abstract

Primordial germ cells (PGCs) are reproductive stem cells that are valuable for avian biobanking and germline transmission. However, their long-term culture remains a challenge beyond a few poultry species. Here, we compare the transcriptomic impact and reproductive viability between short- and long-term chicken and zebra finch gonadal PGC culture protocols. Using single-cell transcriptomics, we identified rapid germline differentiation in zebra finch gonadal cultures compared to stable PGC identity in chicken, highlighting divergent signaling pathway responses to in vitro germline culture between species. These differentiation profiles were similarly found in low-serum zebra finch blood PGC cultures. Nonetheless, injections of these short-term zebra finch gonadal PGC cultures demonstrated sufficient gonadal reconstitution of host embryos to produce zebra finch germline chimeras and donor-derived offspring. This study demonstrates the minimal viability of short-term songbird gonadal cultures, and offers a roadmap to improve the longevity and self-renewal of non-poultry avian PGC cultures.

Indexed as

Cell Culture TechniquesGerm CellsGonadsSongbirdsAnimalsCell DifferentiationCells, CulturedChickensFemaleFinchesMaleTranscriptomeassisted reproductive technologychickengermline chimeragermline transmissiongonadal cultureLRI analysisprimordial germ cell culturescRNAseqsongbirdzebra finch

Identifiers

PMID42624100
PMCPMC13555450

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.