Evidence map›Paper›PMID 42623239›Full record

ArticleSTAR protocols2026

Protocol for manipulating mRNA splicing via vivo-morpholino in early-activating muscle stem cells.

Kangning Lin, Yishu Yin, Tom H Cheung

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kangning LinDivision of Life Science, The Hong Kong University of Science and Technology (HKUST), Hong Kong, China; Hong Kong Center for Neurodegenerative Diseases, InnoHK, Hong Kong, China. Electronic address: kangninglin@ust.hk.
Yishu YinDivision of Life Science, The Hong Kong University of Science and Technology (HKUST), Hong Kong, China.
Tom H CheungDivision of Life Science, The Hong Kong University of Science and Technology (HKUST), Hong Kong, China; Center for Stem Cell Research, State Key Laboratory of Nervous System Disorders, Daniel and Mayce Yu Molecular Neuroscience Center, SIAT-HKUST Joint Laboratory for Brain Science, The Hong Kong University of Science and Technology (HKUST), Hong Kong, China; Hong Kong Center for Neurodegenerative Diseases, InnoHK, Hong Kong, China; Guangdong Provincial Key Laboratory of Brain Science, Disease and Drug Development, Shenzhen-Hong Kong Institute of Brain Science, HKUST Shenzhen Research Institute, Shenzhen, China. Electronic address: tcheung@ust.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Muscle stem cells (MuSCs), also called satellite cells (SCs), are essential for skeletal muscle regeneration. SCs undergo rapid splicing changes upon activation, hindering the study of alternative splicing during SC activation. Here, we describe a protocol for manipulating mRNA splicing in early-activating SCs during isolation using vivo-morpholino (Vivo-MO). We also detail steps for isolating vivo-morpholino-treated SCs, validating the splicing alteration by RT-PCR, and evaluating the functional outcome in SC activation using an EdU incorporation assay. For complete details on the use and execution of this protocol, please refer to Lin et al.

Indexed as

Cell BiologyCell isolationFlow CytometryMolecular BiologyStem Cells

Identifiers

PMID42623239
PMCPMC13521226

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.