Evidence map›Paper›PMID 42623236›Full record

ArticleSTAR protocols2026

Protocol for identifying glycosylated RNAs using solid-phase chemical conjugation and glycosidase release.

Jianbo Deng, Xinyu Miao, Xiaotong Wang, Jiajia Li, Shengye Wen, Zeyang Zhou, Yu Gao, Junhong Jiang, Xiaodong Yang, Shuang Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jianbo DengDepartment of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Laboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University of Medical College, Shantou, Guangdong 515041, China; Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
Xinyu MiaoLaboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University of Medical College, Shantou, Guangdong 515041, China; Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
Xiaotong WangDepartment of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China; Department of Hepatology and Gastroenterology, The Affiliated Infectious Hospital of Soochow University, Suzhou, Jiangsu 215004, China; National Center of Technology Innovation for Biopharmaceuticals, Suzhou, Jiangsu 215000, China.
Jiajia LiLaboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University of Medical College, Shantou, Guangdong 515041, China; Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
Shengye WenLaboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University of Medical College, Shantou, Guangdong 515041, China; Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
Zeyang ZhouDepartment of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China; National Center of Technology Innovation for Biopharmaceuticals, Suzhou, Jiangsu 215000, China.
Yu GaoLaboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University of Medical College, Shantou, Guangdong 515041, China; Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
Junhong JiangDepartment of Respiratory Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215123, China.
Xiaodong YangDepartment of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; National Center of Technology Innovation for Biopharmaceuticals, Suzhou, Jiangsu 215000, China. Electronic address: yangxiaodong2366@suda.edu.cn.
Shuang YangDepartment of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Laboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University of Medical College, Shantou, Guangdong 515041, China; Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China; Department of Respiratory Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215123, China. Electronic address: jake.yang@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA glycosylation plays a critical role in cellular signaling. Here, we present a protocol for enriching glycoRNAs from cultured cells and clinical tissues using a solid-phase chemoenzymatic technique. We describe steps for employing galactose oxidase (GAO) to selectively oxidize terminal sugar residues, enabling covalent capture on hydrazide resin, followed by enzymatic release using PNGase F. This scalable and robust assay efficiently enriches N-linked glycoRNAs, facilitating the systematic characterization of disease-specific RNA glycosylation patterns.

Indexed as

Cell BiologyChemistryClinical ProtocolMolecular/Chemical ProbesSystems biology

Identifiers

PMID42623236
PMCPMC13521237

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.