ArticleFrontiers in microbiology2026
Bactericidal and cell membrane disruption potential of protein extract from buttermilk-derived probiotic
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Foodborne Salmonellosis remains a significant global health challenge, and the rise of antimicrobial resistance is limiting the efficacy of traditional antibiotic treatments, emphasising the need to develop natural biopreservatives to improve food safety. The present study evaluates the antimicrobial potential of a bioactive protein fraction isolated from the probiotic isolate Methods: Antimicrobial protein was extracted from Results: The protein extract showed dose-dependent reduction in pathogen growth with the minimum inhibitory concentration. Growth kinetics and viable cell count revealed a rapid bactericidal effect of the protein extract. The mode of action of the extract involved changes in the membrane structure of pathogen cells, causing metabolic disruption and cellular homeostatic imbalance, as evidenced by the progressive release of intracellular molecules. Furthermore, alterations in cellular structure and function induced by the protein extract were investigated using scanning electron microscopy and analysis of protein expression patterns in treated Discussion: Together, these findings demonstrate a multitargeted antimicrobial mechanism of the bioactive protein fraction from
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