Evidence map›Paper›PMID 42619757›Full record

ArticlebioRxiv : the preprint server for biology2026

A nuclear targeting approach enables efficient transgenesis in the milkweed bug

Yu Shirai, Yousuf Hashmi, Ashley Watts, Jonchee A Kao, Cassandra G Extavour

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yu ShiraiDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.ORCID 0009-0009-0346-7873
Yousuf HashmiDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.ORCID 0009-0009-4486-1922
Ashley WattsDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
Jonchee A KaoDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.ORCID 0000-0003-1701-3265
Cassandra G ExtavourDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.ORCID 0000-0003-2922-5855

Funding

Molecular mechanisms of cell fate determinant assemblyR01GM143611 · NIGMS · HARVARD UNIVERSITY · PI EXTAVOUR, CASSANDRA G · 2022 to 2025
$1.9M
NIGMS NIH HHS R01 GM143611
6 · The paper itself

Abstract

Insects show extreme diversity and have long intrigued biologists. Recent technological advancements, such as gene editing and transgenesis, should in principle enable the use of almost any insect species for biological research. However, in practice, species-specific challenges remain and conditions must be optimized carefully. Here, using the milkweed bug

Indexed as

CRISPR/Cas9Oncopeltus fasciatuspiggyBacQ-systemReporter assayTransgenesis

Identifiers

PMID42619757
PMCPMC13484257

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.