ArticleEMBO molecular medicine2026
Methyltransferase-assisted single duplex sequencing for detecting circulating tumor DNA.
Article in EMBO molecular medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
9 authors.
Funding
Abstract
Accurate circulating tumor DNA (ctDNA) detection is limited by high sequencing errors. To address this, we developed Methyltransferase-Assisted Single Duplex sequencing (MASD-seq), which physically links Watson and Crick strands to enable error correction using double-stranded information within a single read pair. MASD-seq employs enzymatic methyl-sequencing to disrupt strand pairing and uses CpG methyltransferase pre-methylation to enable detection of CpG>TpG and transversion mutations in DNA duplexes. In parallel, MspI digestion enriches tumor CpG>TpG mutations and reduces genome complexity. MASD-seq achieves error rates of 5.4 × 10
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Registered trials
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