ArticleAnalytical and bioanalytical chemistry2026
Graphdiyne oxide as a high-capacity biointerface for multiplexed electrochemical immunosensing of neutrophil biomarkers.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The rapid and reliable quantification of inflammatory biomarkers is highly desirable for clinical diagnostics and point-of-care testing. In this work, we have developed a dual electrochemical immunosensing platform based on graphdiyne oxide (GDYO) for simultaneously determining the neutrophil activation biomarkers CD63 and CD66b. GDYO was integrated onto screen-printed carbon electrodes to create a high-capacity biointerface with an abundance of reactive sites for antibody immobilization and efficient signal transduction. The analytical approach relied on a sandwich immunoassay configuration that employed horseradish peroxidase (HRP)-labeled secondary antibodies. Amperometric detection was performed using the hydroquinone/hydrogen peroxide redox system. Under optimized conditions, the platform enabled the simultaneous quantification of both analytes within a concentration range of 0.005-100 ng·mL
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