Evidence map›Paper›PMID 42615605›Full record

ArticlemSphere2026

Elevated temperature undermines the efficacy of

Lili M Kim, Byoung-Mo Koo, Carol A Gross, Horia Todor

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lili M KimDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0003-3875-2542
Byoung-Mo KooDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.
Carol A GrossDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0002-5595-9732
Horia TodorDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0001-5556-6085

Funding

Cellular homeostasis pathways in bacteriaR35GM118061 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GROSS, CAROL ANNE · 2016 to 2025
$9.0M
NIGMS NIH HHS R35 GM118061NIH HHS R35 GM118061
6 · The paper itself

Abstract

Targeted gene repression using CRISPRi has become a foundational technique for microbiology. The effector protein in most CRISPRi systems is a catalytically inactivated version of Cas9, an RNA-guided DNA endonuclease from IMPORTANCE: CRISPRi is a common technique for inducible gene depletion in bacteria and has been used for many different types of experiments in increasingly diverse bacteria. We identify increased temperatures as an important limitation of the most common CRISPRi effector (

Indexed as

CRISPR-Associated Protein 9CRISPR-Cas SystemsHot TemperatureStreptococcus pyogenesTemperatureBacillus subtilisCRISPR-Associated Protein 9Bacillus subtilisCRISPRiheat shock

Identifiers

PMID42615605
PMCPMC13621834

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.