ArticleFrontiers in microbiology2026
Application of mNGS in traceability investigation of foodborne disease outbreaks caused by
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Methods: Clinical, food, and environmental surface swab samples were collected. The clinical samples were screened using the 14-plex PCR assay for rapid pathogen detection. Metagenomic sequencing (mNGS) was performed on all samples in parallel. Conventional bacterial culture was also conducted, and the obtained isolates were subjected to whole-genome sequencing (WGS). A SNP-based phylogenetic tree was constructed using WGS data from clinical isolates and reference strains from different geographical regions. Results: This foodborne outbreak was caused by Conclusions: Our integrated approach combining culture, WGS, and mNGS proved effective for rapid outbreak traceability, suggesting that the outbreak most likely originated from a contaminated food source associated with cross-regional dissemination, although the specific vehicle and transmission route remain to be determined.
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