Evidence map›Paper›PMID 42613586›Full record

ArticleThe New phytologist2026

Evolutionary origin of a new gene drives root growth in one desert poplar.

Chengyu Gao, Xiaotao Bai, Qianqian Wei, Xuemin Shao, Pan Zhang, Qi Zhang, Junjie Wang, Dongshi Wan, Jianquan Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The New phytologist, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chengyu Gao *State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.
Xiaotao Bai *State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.
Qianqian WeiState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.
Xuemin ShaoState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.
Pan ZhangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.
Qi ZhangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.
Junjie WangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.
Dongshi WanState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.ORCID https://orcid.org/0000-0002-6528-0352
Jianquan LiuState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, 730000, China.ORCID https://orcid.org/0000-0002-4237-7418

Funding

Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China YB2025XDXM910Gansu Provincial Top-leading Talents ProgramNational Natural Science Foundation of China 31870580National Natural Science Foundation of China 32471894
6 · The paper itself

Abstract

Populus euphratica develops an extensive root system that supports survival in arid environments. We identified PeuPLDζ4, a new gene arising from tandem duplication in P. euphratica, and evaluated its effects on root growth and drought-related performance. Phylogenetic and promoter-reporter analyses, enzyme assays, and subcellular localization characterized PeuPLDζ4 divergence. Its effects were evaluated in transgenic Arabidopsis thaliana, P. alba var. pyramidalis, wheat, and rice. Phosphatidic acid (PA) and hormone measurements, reporter imaging, transcriptomics, protein-lipid overlay, dual-luciferase, and electrophoretic mobility shift assays investigated the mechanism. Compared with its paralog PeuPLDζ3, PeuPLDζ4 showed drought-inducible root expression, higher enzymatic activity, and additional nuclear-associated localization. PeuPLDζ4 overexpression promoted root growth across species and was associated with increased PA accumulation in root-tip tissues, elevated auxin (IAA) and cytokinin (CK) contents, enhanced IAA and CK reporter responses, and an increased IAA : CK ratio. NAC097 was identified as a PA-associated candidate regulator that bound and activated the LONELY GUY 2 (LOG2) and IIOPENTENYLTRANSFERASE 4 (IPT4) promoters. These findings indicate that functional divergence of a newly duplicated PLDζ gene contributed to root-related adaptation in P. euphratica. The PA-associated NAC097-LOG2/IPT4 branch provides a candidate link between lipid signaling and CK regulation, while PeuPLDζ4 may offer a resource for improving drought-related traits in crops.

Indexed as

Desert ClimateEvolution, MolecularGenes, PlantPlant RootsPopulusArabidopsisCytokininsDrought ResistanceDroughtsGene DuplicationGene Expression Regulation, PlantIndoleacetic AcidsPhosphatidic AcidsPhylogenyPlant Growth RegulatorsPlant ProteinsCytokininsIndoleacetic AcidsPhosphatidic AcidsPlant Growth RegulatorsPlant Proteinsauxin and cytokinindrought adaptationneofunctionalizationnew genePeuPLDζ4phosphatidic acid (PA)Populus euphratica

Identifiers

PMID42613586
PMCPMC13583415

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.