A 3D Tumor Spheroid Platform to Model Fusobacterium nucleatum-Induced Tumorigenesis.

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yi-Wei ChenDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California - Los Angeles, Los Angeles, CA, USA. yiweichen515@ucla.edu.ORCID https://orcid.org/0000-0001-7746-6026

Omri KarivDepartment of Microbiology, Immunology and Molecular Genetics, University of California - Los Angeles, Los Angeles, CA, USA.ORCID https://orcid.org/0009-0003-9306-4563

Hung Ton-ThatDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California - Los Angeles, Los Angeles, CA, USA.ORCID https://orcid.org/0000-0003-1611-0469

Funding

Assembly and function of outer membrane tubules in Fusobacterium nucleatumR01DE033900 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Hung Ton-That · 2024 to 2026

$1.4M

NIDCR NIH HHS R01 DE033900

6 · The paper itself

Abstract

Cancer cell line spheroids provide a three-dimensional (3D) culture platform that enables the evaluation of cancer cell aggregation, tumor initiation, cell proliferation, and overall tumor growth. Compared with conventional two-dimensional monolayer cultures, spheroid models more faithfully recapitulate the architectural, physiological, and microenvironmental features of solid tumors, making them particularly well suited for studying tumor biology and microbe-host interactions. Previous work has leveraged this 3D system to investigate the role of the oral oncobacterium Fusobacterium nucleatum in promoting tumorigenesis. Here, we present a detailed protocol in which colorectal cancer cells are seeded into ultralow attachment plates and co-cultured with F. nucleatum either prior to spheroid formation, to model early tumor development, or after spheroids have been established, to model tumor progression. This assay provides a tractable platform for dissecting cancer cell-microbe interactions and supports a contributory role for F. nucleatum in colorectal tumor development.

Indexed as

CarcinogenesisCell Culture Techniques, Three DimensionalColorectal NeoplasmsFusobacterium InfectionsFusobacterium nucleatumSpheroids, CellularCell Line, TumorCell ProliferationCoculture TechniquesHumansColorectal cancerFusobacterium nucleatumSpheroid formationSpheroid growthVirulence

Identifiers

PMID42613572

DOI10.1128/mbio.01032-25

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.