Evidence map›Paper›PMID 42613569›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Isolation and Visualization Fusobacterium nucleatum Microcompartments.

Dana Franklin, Angela Agnew, John Crow, Z Hong Zhou, Hung Ton-That

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dana Franklin *Molecular Biology Institute, University of California - Los Angeles, Los Angeles, CA, USA.
Angela Agnew *Molecular Biology Institute, University of California - Los Angeles, Los Angeles, CA, USA.
John CrowMolecular Biology Institute, University of California - Los Angeles, Los Angeles, CA, USA.
Z Hong ZhouMolecular Biology Institute, University of California - Los Angeles, Los Angeles, CA, USA.
Hung Ton-ThatMolecular Biology Institute, University of California - Los Angeles, Los Angeles, CA, USA. htonthat@dentistry.ucla.edu.ORCID https://orcid.org/0000-0003-1611-0469

Funding

UCLA Dentist-Scientist and Oral Health-Researcher Training ProgramT90DE030860 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TON-THAT, HUNG, WONG, DAVID T · 2021 to 2025
$2.1M
Metabolic modulation of Fusobacterium nucleatum virulenceR21DE032906 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TON-THAT, HUNG · 2023 to 2024
$605k
NIDCR NIH HHS R21 DE032906NIDCR NIH HHS T90 DE030860
6 · The paper itself

Abstract

In Fusobacterium nucleatum, bacterial microcompartments (BMCs) are polyhedral organelles that encapsulate the catabolism of ethanolamine (EA), creating a specialized environment that protects the rest of the cell from toxic metabolic byproducts. In this chapter, we present a protocol for the isolation and visualization of F. nucleatum BMCs with transmission electron microscopy (TEM), cryogenic electron microscopy (cryoEM), and cryogenic electron tomography (cryoET). The protocol includes anaerobic cultivation of F. nucleatum, a strategy for BMC purification, sample preparation and image acquisition for TEM, cryoEM, and cryoET, followed by 3D reconstruction and visualization. These approaches are adaptable to BMCs from other bacterial species, offering a robust framework for structural and functional studies of diverse BMC systems.

Indexed as

Fusobacterium nucleatumOrganellesCryoelectron MicroscopyElectron Microscope TomographyEthanolamineImaging, Three-DimensionalMicroscopy, Electron, TransmissionEthanolamineBacterial microcompartmentsCryogenic electron microscopyElectron microscopyEthanolamineFusobacterium nucleatum

Identifiers

PMID42613569

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.