ArticleMethods in molecular biology (Clifton, N.J.)2026
Tracking Surface Protein Assembly and Peptidoglycan Biosynthesis in Fusobacterium nucleatum.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Fusobacterium nucleatum is a Gram-negative, obligate anaerobe and oncogenic oral bacterium that employs a diverse array of surface-exposed adhesins to colonize extra-oral tissues. Among these, Fusobacterial adhesin A (FadA) is a well-characterized virulence factor known for its ability to target epithelial and endothelial tight junctions, disrupt barrier integrity, and activate pro-tumorigenic signaling pathways implicated in colorectal cancer. FadA also facilitates placental and fetal colonization, contributing to adverse pregnancy outcomes such as preterm birth and stillbirth. While its functional roles in bacterial colonization and pathogenicity are increasingly recognized, the spatial and temporal dynamics of FadA assembly on the bacterial surface-and its relationship to cell wall biogenesis-remain poorly understood. Here, we present a fluorescence-based labeling approach using fluorescent D-amino acids (FDAAs) to simultaneously track peptidoglycan biosynthesis and the localization of FadA in F. nucleatum. This methodology provides a versatile platform for investigating the dynamic interplay between virulence factor localization and cell envelope remodeling.
Indexed as
Identifiers
42613566What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.