Evidence map›Paper›PMID 42613543›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Intracerebroventricular Administration of Gangliosides for In Vivo Functional Studies in Mice.

John Monyror, Melanie Horkey, Asifa K Zaidi, Simonetta Sipione

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

John MonyrorDepartment of Pharmacology and Glycomics Institute of Alberta, University of Alberta, Edmonton, AB, Canada.
Melanie HorkeyDepartment of Pharmacology, University of Alberta, Edmonton, AB, Canada.
Asifa K ZaidiDepartment of Pharmacology and Glycomics Institute of Alberta, University of Alberta, Edmonton, AB, Canada.
Simonetta SipioneDepartment of Pharmacology, Neuroscience and Mental Health Institute and Glycomics Institute of Alberta, University of Alberta, Edmonton, AB, Canada. ssipione@ualberta.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Levels of gangliosides are reduced in several neurological conditions, and their restoration has shown therapeutic potential in animal models of Huntington's disease and Parkinson's disease. However, the full range of functions of gangliosides in the brain, along with their molecular targets and mechanisms of action following exogenous administration, remains ill-defined. Due to their poor blood-brain barrier (BBB) permeability, gangliosides face significant challenges when delivered through non-invasive routes. Direct intracerebroventricular (ICV) infusion circumvents this issue by bypassing the BBB, ensuring a more precise delivery to brain structures and eliminating uncertainties related to brain penetration. Here, we describe a protocol for ICV infusion of gangliosides in mice that enables in vivo pre-clinical studies of their effects, while eliminating uncertainties about the extent of drug delivery to the brain through the BBB.

Indexed as

GangliosidesAnimalsBlood-Brain BarrierBrainDisease Models, AnimalHuntington DiseaseInfusions, IntraventricularInjections, IntraventricularMiceGangliosidesGM1Huntington’s diseaseNeurodegenerationOsmotic minipumpsStereotaxic surgery

Identifiers

PMID42613543

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.