Evidence map›Paper›PMID 42613537›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Flow Cytometry Analysis of Gangliosides Expressed on Extracellular Vesicles.

Yuhsuke Ohmi, Hiroka Koyanagi, Mohammad Abul Hasnat, Keiko Furukawa

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yuhsuke OhmiDepartment of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Aichi, Japan. ooumi82@fsc.chubu.ac.jp.
Hiroka KoyanagiDepartment of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Aichi, Japan.
Mohammad Abul HasnatDepartment of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Aichi, Japan.
Keiko FurukawaDepartment of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Aichi, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In flow cytometry analysis, molecules on cells can be detected by labeling with antibodies or other reagents. However, extracellular vesicles (EVs) released by cells are of a small particle size, making it difficult to detect molecules on EVs using conventional flow cytometry. Here, we describe a method for isolating EVs secreted by cells and report a flow cytometry analysis for gangliosides on EVs. By using Tim4-conjugated beads, which bind specifically to EVs, molecules expressed on EVs can be easily detected even with flow cytometry.

Indexed as

Extracellular VesiclesFlow CytometryGangliosidesAnimalsHumansGangliosidesExtracellular vesiclesFlow cytometryGangliosideGD2GD3Tim4

Identifiers

PMID42613537

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.