Evidence map›Paper›PMID 42613534›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Small-Scale Cell Biological Analysis of Lipid Rafts of Blood Platelets and Cerebellar Neurons.

Keisuke Komatsuya, Norihito Kikuchi, Tetsuya Hirabayashi, Kohji Kasahara

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Keisuke KomatsuyaBiomembrane Group, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Norihito KikuchiBiomembrane Group, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Tetsuya HirabayashiBiomembrane Group, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Kohji KasaharaBiomembrane Group, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan. kasahara-kj@igakuken.or.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipid rafts are dynamic assemblies of glycosphingolipids, sphingomyelin, cholesterol, and proteins that can be stabilized into platforms involved in the regulation of various vital cellular processes. Here, we describe small-scale isolation methods of lipid rafts. Detergent-resistant membrane raft fraction is isolated by a volume of 0.8 mL sucrose density gradient centrifugation from blood platelets. Raft fraction is also isolated from cerebellar neurons by co-immunoprecipitation using anti-glycosphingolipid monoclonal antibodies.

Indexed as

Blood PlateletsCell FractionationCerebellumMembrane MicrodomainsNeuronsAnimalsCentrifugation, Density GradientGlycosphingolipidsHumansImmunoprecipitationSphingomyelinsGlycosphingolipidsSphingomyelinsCerebellar neuronsDetergent-resistant membraneGlycosphingolipidsImmunoprecipitationLipid raftsMass spectrometryPlateletsSignal transductionSphingomyelinSucrose density gradient

Identifiers

PMID42613534

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.