Evidence map›Paper›PMID 42613523›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Chemical Identification of Glycosphingolipid Antigens Recognized by Antibodies.

Koichi Furukawa, Yuhsuke Ohmi, Orie Tajima, Keiko Furukawa

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Koichi FurukawaDepartment of Biomedical Sciences, Chubu University College of Life and Health Sciences, Kasugai, Aichi, Japan. koichi@fsc.chubu.ac.jp.
Yuhsuke OhmiDepartment of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Aichi, Japan.
Orie TajimaDepartment of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Aichi, Japan.
Keiko FurukawaDepartment of Biomedical Sciences, Chubu University College of Life and Health Sciences, Kasugai, Aichi, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A number of monoclonal antibodies have been generated aiming to detect markers for tissue development and degeneration, cell differentiation, and malignant transformation. Among antigens recognized by these monoclonal antibodies, glycosphingolipids including gangliosides are included. In this chapter, fundamental methods for chemical identification of ganglioside antigens recognized by antibodies are summarized. After extraction of lipids from cells and tissues using organic solvent, neutral and acidic glycosphingolipids are separated with/without pre-elimination of phospholipids. Extracted glycolipids are applied for thin-layer chromatography combined with immunodetection to identify specific antigen component recognized by the antibody. To finally define the antigen structure, mass spectrometry is effectively used. Confirmation with transfection of supposed glycosyltransferase cDNA has also been a supportive way.

Indexed as

Antibodies, MonoclonalAntigensGlycosphingolipidsAnimalsChromatography, Thin LayerGangliosidesHumansImmunoblottingMass SpectrometryAntibodies, MonoclonalAntigensGangliosidesGlycosphingolipidsEvaporationGlycosphingolipidLipid extractionMonoclonal antibodyThin-layer chromatographyTLC-immunoblotting

Identifiers

PMID42613523

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.