Evidence map›Paper›PMID 42611700›Full record

ArticleSTAR protocols2026

Protocol for multimodal programming of murine macrophage phenotypes.

Gauri Mirji, Sajad Ahmad Bhat, Rahul S Shinde

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Gauri MirjiMolecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center, The Wistar Institute, Philadelphia, PA 19104, USA. Electronic address: gmirji@wistar.org.
Sajad Ahmad BhatMolecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center, The Wistar Institute, Philadelphia, PA 19104, USA. Electronic address: sbhat@wistar.org.
Rahul S ShindeUSF Center for Microbiome Research, Microbiomes Institute, Department of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. Electronic address: rsshinde@usf.edu.

Funding

Characterizing the sources, mechanisms, and translational relevance of microbial TMAO in driving anti-tumor immunity in pancreatic cancer.R37CA280869 · NCI · WISTAR INSTITUTE · PI Rahul Suresh Shinde · 2024 to 2026
$1.7M
Investigating BCAA oxidation and signaling in macrophages to drive anti-tumor immunity in PDACR01CA293027 · NCI · WISTAR INSTITUTE · PI Rahul Suresh Shinde · 2026 to 2026
$781k
The role of gut microbe-derived choline metabolites in driving the pro-inflammatory macrophage phenotype and restricting pancreatic cancerR21CA259240 · NCI · WISTAR INSTITUTE · PI SHINDE, RAHUL SURESH · 2021 to 2022
$477k
NCI NIH HHS R01 CA293027NCI NIH HHS R21 CA259240NCI NIH HHS R37 CA280869
6 · The paper itself

Abstract

Macrophages continuously sense their environment and adopt distinct functional states in response to danger, repair, and homeostatic signals. Here, we present a protocol for generating murine bone marrow-derived macrophages and programming them with microbial/TLR and nucleic acid-sensing agonists, IL-4, apoptotic cells, and tumor microenvironment analogs. We describe steps for stimulation conditions and downstream immunological assays, including flow cytometry, ELISA, RT-PCR, and multiomics, to comprehensively profile murine macrophage phenotypes and functions across these contexts. For complete details on the use and execution of this protocol, please refer to Mirji et al.

Indexed as

CancerImmunologyMetabolism

Identifiers

PMID42611700
PMCPMC13519604

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.