ArticleSTAR protocols2026
Protocol for flash-freezing, pooling, and preparing single nuclei suspensions from amniote inner-ear sensory epithelia.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The inner-ear sensory epithelia are characteristically difficult to dissociate into healthy single-cell suspensions, with adult tissue presenting the most challenges. Recent advances in RNA sequencing have allowed for transcriptomic profiling of single nuclei, instead of single cells, offering a valuable alternative for processing sensitive tissue for single-cell genomics. Here, we provide a detailed protocol for flash-freezing, pooling, and preparing single nuclei suspensions from mouse and chicken inner-ear sensory epithelia, with a focus on optimization and quality control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.