Evidence map›Paper›PMID 42610705›Full record

ArticleMicrobiology spectrum2026

Adaptation of the molecular bacterial load assay for real-time quantification of

B Andersson, V Kholod, M Jonsson Nordvall, C Braian, R Kumar, K Niward, T Schön

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

B AnderssonDepartment of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, Linköping, Sweden.ORCID 0000-0002-9562-0872
V KholodDepartment of Clinical Microbiology, Linköping University Hospital, Linköping, Sweden.
M Jonsson NordvallDepartment of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, Linköping, Sweden.
C BraianDepartment of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, Linköping, Sweden.ORCID 0000-0003-1784-2864
R KumarDepartment of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, Linköping, Sweden.
K NiwardDepartment of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, Linköping, Sweden.
T SchönDepartment of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, Linköping, Sweden.

Funding

ALF Östergötland ALF-987589ALF Östergötland RÖ-1005642Medical Research Council of Southeast Sweden 968810, 101639Swedish Heart Lung Foundation 20220148Swedish Heart Lung Foundation 20220238, 20251638Swedish Research Council 2022-00865
6 · The paper itself

Abstract

Rapid quantification of IMPORTANCE: The current tuberculosis molecular bacterial load assay (TB-MBLA) protocol relies on toxic chemicals and manual RNA extraction, making the procedure both time‑consuming and labor‑intensive. We optimized and validated a faster TB-MBLA strategy, which can be adapted for routine clinical laboratories, including inactivation by heating, to facilitate implementation in settings without a BSL3 laboratory.

Indexed as

Bacterial LoadMycobacterium tuberculosisTuberculosisHumansRNA, BacterialRNA, Ribosomal, 16SSensitivity and SpecificitySputumRNA, BacterialRNA, Ribosomal, 16Sautomated RNA extractionheat inactivationmolecular bacterial load assayMycobacterium tuberculosisTB-MBLA

Identifiers

PMID42610705
PMCPMC13689229

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.