Evidence map›Paper›PMID 42610650›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Protease-Triggered Chromogenic Release From Peptide-Modified Calcium Carbonate Microspheres Enables Colorimetric Detection of Porphyromonas gingivalis Activity.

Yuki Hiruta, Zhicheng Jin, Mari Adachi, Lubna Amer, Natalia O Tjokro, Diego F Trujillo, Jan Potempa, Anthony J O'Donoghue, Casey Chen, Jesse V Jokerst

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuki HirutaAiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California San Diego, California, USA.ORCID https://orcid.org/0000-0001-7303-4189
Zhicheng JinAiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California San Diego, California, USA.ORCID https://orcid.org/0000-0001-6072-7533
Mari AdachiSchool of Biological Sciences, Molecular and Cell Biology, University of California San Diego, California, USA.
Lubna AmerProgram in Materials Science and Engineering, University of California San Diego, California, USA.ORCID https://orcid.org/0000-0001-5715-5060
Natalia O TjokroHerman Ostrow School of Dentistry, University of Southern California, Los Angeles, USA.
Diego F TrujilloSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, California, USA.
Jan PotempaDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
Anthony J O'DonoghueSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, California, USA.ORCID https://orcid.org/0000-0001-5695-0409
Casey ChenHerman Ostrow School of Dentistry, University of Southern California, Los Angeles, USA.
Jesse V JokerstAiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California San Diego, California, USA.ORCID https://orcid.org/0000-0003-2829-6408

Funding

A Chairside Diagnostic to Reduce the Need for Endodontic RetreatmentR21DE035344 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JOKERST, JESSE VINCENT · 2025 to 2025
$443k
Molecular Imaging of Gingipain Activity in Advanced PeriodontitisR21DE029917 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JOKERST, JESSE VINCENT · 2020 to 2021
$406k
Fukuzawa Memorial FundJapan Society for the Promotion of ScienceNational Nanotechnology Coordinated InfrastructureNational Science Foundation ECCS-2025752NIDCR NIH HHS R21 DE029917NIDCR NIH HHS R21 DE035344Promotion of Joint International Research (Fostering Joint International Research 21K06495Promotion of Joint International Research (Fostering Joint International Research 22KK0234Shiley Foundation
6 · The paper itself

Abstract

Porphyromonas (P.) gingivalis is a Gram-negative anaerobic bacterium with exceptionally high pathogenicity among periodontal pathogens. It is associated not only with periodontal disease but also with various systemic diseases, including Alzheimer's disease, rheumatoid arthritis, and atherosclerosis. Therefore, early detection of this bacterium is crucial for enabling subsequent treatment and prevention of such conditions. Herein, we report a chromogenic release-based assay system utilizing calcium carbonate microspheres and a dye-labeled peptide that is selectively cleaved by an Arg-specific protease called gingipain (RgpB) that is secreted by P. gingivalis. By utilizing the reactive anhydride functional groups of poly(isobutylene-alt-maleic anhydride), we simultaneously achieved the conjugation of the dye-labeled peptide and its immobilization onto calcium carbonate microspheres. Through optimization of the peptide sequence and the surface modification density on calcium carbonate microspheres, the assay of RgpB detection with the absorbance of supernatant exhibited a low limit of detection of 0.25 nM, as well as high storage stability and selectivity for P. gingivalis. The measurements obtained with this assay system for clinical gingival crevicular fluid samples showed a high correlation (Pearson's r = 0.79) with qPCR results. This assay system offers an on-site, visual means to monitor P. gingivalis activity without requiring specialized instrumentation.

Indexed as

biosensorscalcium carbonatechromogenic release‐based assaypeptidesP. gingivalis

Identifiers

PMID42610650
PMCPMC13483131

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.