Three-color high-definition whole-brain imaging: simultaneous analysis of multiple anatomical structures with colocalized cytoarchitectural information.

Tao Jiang, Miao Ren, Xueyan Jia, Jing Yuan, Xiangning Li, Qingming Luo, Hui Gong

Abstract read

In one paragraph

Article in Biomedical optics express, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tao JiangHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, 215123, China.ORCID https://orcid.org/0000-0002-4487-299X

Miao RenState Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Sanya, 570228, China.

Xueyan JiaHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, 215123, China.

Jing YuanHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, 215123, China.ORCID https://orcid.org/0000-0001-9050-4496

Xiangning LiHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, 215123, China.

Qingming LuoState Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Sanya, 570228, China.ORCID https://orcid.org/0000-0002-6725-9311

Hui GongHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, 215123, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Simultaneous multicolor whole-brain imaging with cytoarchitectural reference is critical for neural circuit mapping, but sequential scanning prolongs acquisition and complicates inter-channel registration. We developed a three-color high-definition fluorescent micro-optical sectioning tomography (HD-fMOST) system for three-channel high-throughput imaging of the whole mouse brain. We corrected chromatic aberration in the 405 nm illumination path and designed a precision 6-axis stage for pixel-level registration of three channels. Real-time DAPI counterstaining provides cytoarchitectural landmarks while preserving green/red fluorescence excitation. The system achieves sub-micron voxel resolution (0.32 × 0.32 × 1 μm

Identifiers

PMID42609939

DOI10.1364/BOE.604080

PMCPMC13481074

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.