ArticleBiomedical optics express2026
Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy.
Article in Biomedical optics express, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
We report the development of a novel two-photon excitation stimulated emission depletion microscopy system, namely 2PE-scSTED, featuring a custom light source setup that integrates a supercontinuum (SC) laser light source with our proprietary 1064 nm pulsed semiconductor excitation laser. This SC source provides the capability of emitting a broadband spectral range, enabling highly reliable selection of depletion wavelength. Utilizing 2PE-scSTED, we investigated the depletion characteristics of several fluorophores. By employing a tunable filter to precisely select depletion wavelength from 640 to 800 nm, we systematically measured the depletion efficiency and identified the optimal wavelengths that maximize fluorescence depletion efficiency for four distinct fluorescent probes. This approach yielded super-resolution images with a lateral spatial resolution as fine as 45 nm (approximately
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