Evidence map›Paper›PMID 42608461›Full record

ArticleNature methods2026

PASTA: versatile tyramide-oligonucleotide amplification for multimodal spatial biology.

Hendrik A Michel, Paige McCallum, Wenrui Wu, Huaying Qiu, Jia Le Lee, Shuli Luo, Chi Ngai Chan, Johanna Schaffenrath, Yao Yu Yeo, Stephanie Pei Tung Yiu and 17 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Hendrik A MichelCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Paige McCallumCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0009-0007-9027-3860
Wenrui WuCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Huaying QiuCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Jia Le LeeCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-1600-6437
Shuli LuoCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Chi Ngai ChanCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0001-9106-9481
Johanna SchaffenrathCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Yao Yu YeoCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-7604-2296
Stephanie Pei Tung YiuCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Yang WangCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Lindsay ParmeleeCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-2488-3544
Hongbo WangCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Tania PannelliniDivision of Hematopathology, IEO European Institute of Oncology IRCCS, Milan, Italy.
Melinda BurgessFrazer Institute, Translational Research Institute, University of Queensland, Brisbane, Queensland, Australia.
Nourhan El AhmarDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Zoe Xiaozhu ZhangDepartment of Anatomical Pathology, Singapore General Hospital, Singapore, Singapore.
Colm KeaneFrazer Institute, Translational Research Institute, University of Queensland, Brisbane, Queensland, Australia.
Tony Kiat Hon LimDepartment of Anatomical Pathology, Singapore General Hospital, Singapore, Singapore.
Sabina SignorettiDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Sonia Victoria Del RinconDivision of Clinical and Translational Research, McGill University, Montréal, Quebec, Canada.
Bo ZhaoDivision of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
David R McIlwainDepartment of Microbiology and Immunology, University of Nevada Reno, Reno, NV, USA.ORCID http://orcid.org/0000-0002-2537-0273
Yunhao BaiBroad Institute of Harvard and MIT, Cambridge, MA, USA.
Fei ChenBroad Institute of Harvard and MIT, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-2308-3649
Roberto ChiarleDivision of Hematopathology, IEO European Institute of Oncology IRCCS, Milan, Italy.ORCID http://orcid.org/0000-0003-1564-8531
Sizun JiangCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. sjiang3@bidmc.harvard.edu.ORCID http://orcid.org/0000-0001-6149-3142

Funding

Treating the P13-Kinase/AKTP50CA101942 · NCI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI David McDermott · 2003 to 2026
$53.4M
NHP CoreP01AI177687 · NIAID · BETH ISRAEL DEACONESS MEDICAL CENTER · PI Boris Dominik Juelg · 2023 to 2026
$7.4M
Defining Mechanisms of Viral Persistence in Situ at the Single-Cell LevelR01AI149672 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI ESTES, JACOB D · 2020 to 2024
$4.0M
Spatial-Temporal Dissection of Stratified Host Tissue Responses to Severe acute respiratory syndrome-related coronaviruses in situ to Understand Intra-host PathogenesisDP2AI171139 · NIAID · BETH ISRAEL DEACONESS MEDICAL CENTER · PI Sizun Jiang · 2022 to 2026
$2.3M
Dissecting Orchestrated Immune Responses to Glioblastoma within the Native Tissue Microenvironment to Improve Treatment OutcomesR01NS139479 · NINDS · BETH ISRAEL DEACONESS MEDICAL CENTER · PI VASSILIKI A BOUSSIOTIS, Alain Charest · 2025 to 2026
$1.4M
Bill & Melinda Gates Foundation INV-002704NCI NIH HHS P50 CA101942NIAID NIH HHS DP2 AI171139NIAID NIH HHS P01 AI177687NIAID NIH HHS R01 AI149672NINDS NIH HHS R01 NS139479
6 · The paper itself

Abstract

Spatial proteomics is limited by detection sensitivity, multiplexing and multimodal integration, leaving a gap between discovery and clinical assays. Here we present protein and nucleic acid serial tyramide amplification (PASTA), using horseradish peroxidase-mediated oligonucleotide deposition and cyclical imaging for high-plex, multimodal spatial profiling. Compatible with conjugated antibodies and in situ hybridization, PASTA enables simultaneous protein and RNA codetection from formalin-fixed, paraffin-embedded samples, providing a cost-effective bridge from discovery to clinical validation.

Indexed as

Nucleic Acid Amplification TechniquesOligonucleotidesProteomicsTyramineHorseradish PeroxidaseHumansIn Situ HybridizationRNAHorseradish PeroxidaseOligonucleotidesRNATyramine

Identifiers

PMID42608461
PMCPMC13539481

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.