ArticlePloS one2026
Refining the use of seed balls to support seagrass restoration.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
Seagrass ecosystems are experiencing rapid global decline, and current seed-based restoration efforts are frequently hindered by severe early-life bottlenecks such as decapod predation and bioturbation. To bypass these ecological feedbacks, encasing Zostera marina seeds in protective clay matrices (seed balls) presents a promising, low-cost intermediate technology for scalable deployment. To refine this approach, a mesocosm experiment was conducted to evaluate the physical integrity, germination success, and early shoot development of Z. marina seeds across various clay formulations (fireclay, bentonite, and Kettering loam) and drying treatments. Seed balls were deployed either on the sediment surface or buried, and their performance was evaluated using a hurdle-style generalised additive modelling approach. Results indicated a clear trade-off between structural robustness and biological performance. While naked seeds (70.3% emergence) and buried fireclay balls (67.2% emergence) exhibited the highest overall emergence and post-emergence shoot length, surface-deployed seed balls formulated with higher proportions of bentonite and loam (e.g., 2:4:1 ratio) demonstrated the superior structural integrity necessary for resisting degradation during deployment. However, these denser matrices significantly reduced emergence probabilities and restricted shoot development compared to naked seeds. Formulations lacking loam (1:3) performed the poorest across all biological and structural metrics. Despite reduced emergence relative to buried treatments, surface-deployed seed balls still achieved high viability, outperforming typical field germination rates. Although further field-based refinement is required to test formulations under dynamic hydrodynamic and bioturbation pressures, seed balls offer a scientifically robust, scalable mechanism to bypass early-stage predation and support widespread seascape recovery.
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