ArticleSTAR protocols2026
Protocol for covalent ligand discovery via library-versus-proteome screening.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
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Abstract
Here, we present a dynamic combinatorial library-versus-proteome activity-based protein profiling (DCL-ABPP) workflow for high-throughput covalent ligand discovery. We describe steps for integrating a dynamic combinatorial library with proteome-wide mass spectrometry to generate and screen hundreds of ligands in situ, eliminating the need for pre-synthesis. We detail procedures for two complementary modes: competitive screening, which identifies enzyme inhibitors (e.g., serine hydrolases) through family-wide probe competition; and direct screening, which maps covalent ligand binding sites on cysteines. For complete details on the use and execution of this protocol, please refer to Huang et al.
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