Evidence map›Paper›PMID 42606734›Full record

ArticleMedical microbiology and immunology2026

Kinetics and characterization of extracellular vesicle release from cells infected with a clinical isolate of respiratory syncytial virus A (ON1 genotype).

Matteo Fracella, Valeria Manganelli, Alessandra Pierangeli, Carolina Scagnolari, Manuel Pacchiele, Eleonora Coratti, Roberta Campagna, Gloria Riitano, Federica Fratini, Agostina Longo and 2 more

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Article in Medical microbiology and immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Matteo Fracella *Microbiology and Virology Laboratory, Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy. matteo.fracella@uniroma1.it.
Valeria Manganelli *Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Alessandra PierangeliDepartment of Well-being, Health and Environmental Sustainability (BeSSA), Sapienza University of Rome, Rome, Italy.
Carolina ScagnolariMicrobiology and Virology Laboratory, Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Manuel PacchieleMicrobiology and Virology Laboratory, Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Eleonora CorattiMicrobiology and Virology Laboratory, Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Roberta CampagnaMicrobiology and Virology Laboratory, Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Gloria RiitanoDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Federica FratiniProteomics Core Facility, Istituto Superiore di Sanità (ISS), Rome, Italy.
Agostina LongoDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Maurizio Sorice *Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Guido Antonelli *Microbiology and Virology Laboratory, Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite the recent approval of preventive measures, the disease burden of respiratory syncytial virus (RSV) infection in children and older adults remains significant. To improve understanding of RSV disease, this study characterized extracellular microvesicles (EMVs) secreted during in vitro infection with a clinical RSV-A (ON1 genotype) strain (RSV/40G-A), isolated in 2022 from a pediatric pneumonia case. The type and content of EMVs released by A549 and BEAS-2B respiratory epithelial cells infected with this clinical isolate were analyzed up to 96 h post-infection (hpi). RSV infection induced a time-dependent increase in EMV levels compared to uninfected controls. EMVs released by infected BEAS-2B and A549 cells contained increasing levels of RSV nucleocapsid protein (RSV-NP) and viral RNA with a peak at 72 hpi. These findings suggest that the kinetics of vesicular release and EMV content are modulated during RSV infection. EMV secretion by host cells may constitute a potential mechanism for intercellular communication or immune activation.

Indexed as

Epithelial CellsExtracellular VesiclesRespiratory Syncytial Virus, HumanRespiratory Syncytial Virus InfectionsA549 CellsCell LineGenotypeHumansKineticsNucleocapsid ProteinsRNA, ViralNucleocapsid ProteinsRNA, ViralExtracellular vesiclesMicrovesiclesRespiratory epithelial cellsRSV

Identifiers

PMID42606734
PMCPMC13481564

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