Evidence map›Paper›PMID 42606277›Full record

ArticleProtein science : a publication of the Protein Society2026

MAXTIA: A high-throughput platform for rapid functional epitope mapping by kinetic screening of mutant libraries.

Kihoon Kim, Ryo Matsunaga, Takanori Yokoo, Makoto Nakakido, Kouhei Tsumoto

Abstract read
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In one paragraph

Article in Protein science : a publication of the Protein Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kihoon KimDepartment of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.ORCID 0009-0007-6208-9142
Ryo MatsunagaDepartment of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.ORCID 0000-0001-7702-9176
Takanori YokooDepartment of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.
Makoto NakakidoDepartment of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.ORCID 0000-0003-0328-9914
Kouhei TsumotoDepartment of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.ORCID 0000-0001-7643-5164

Funding

Japan Agency for Medical Research and Development JP223fa627001Japan Agency for Medical Research and Development JP223fa727002Japan Science and Technology Agency JPMJAX222IJapan Society for the Promotion of Science London 22K21343Ministry of Education, Culture, Sports, Science and Technology JPMXP1122714694
6 · The paper itself

Abstract

Accurate identification of epitope residues is essential for developing biopharmaceuticals and understanding the mechanisms of immune recognition. However, experimental approaches for residue-level epitope mapping remain time-consuming and labor-intensive, while accurate computational prediction of protein-protein interfaces remains challenging. Here, we present MAXTIA, a high-throughput kinetic screening platform that integrates cell-free protein synthesis with high-throughput surface plasmon resonance and demonstrate its application to alanine scanning-based functional epitope mapping. This workflow enables the rapid preparation and kinetic characterization of up to 384 protein variants, allowing the identification of functional epitope residues within 3 days while simultaneously providing binding affinity and kinetic parameters (K

Indexed as

C-Reactive ProteinEpitope MappingEpitopesHigh-Throughput Screening AssaysKineticsModels, MolecularMutationSurface Plasmon ResonanceC-Reactive ProteinEpitopescell‐free protein synthesisepitope mappinghigh‐throughput experimental systeminteraction analysiskinetic screeningprotein engineering

Identifiers

PMID42606277
PMCPMC13479966

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.