ArticleScientific reports2026
Development and validation of dispersive liquid-liquid microextraction coupled with LC-MS/MS for plasma 5-hydroxyindoleacetic acid quantification with application as a biomarker in major depressive disorder.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A sensitive dispersive liquid-liquid microextraction (DLLME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for plasma 5-hydroxyindoleacetic acid (5-HIAA) quantification as a depression biomarker. Box-Behnken design evaluated four DLLME factors (disperser volume, extraction volume, pH, centrifugation time) where second-order polynomial model identified optimal conditions with R² = 0.9444, achieving 95.1% extraction recovery. Chromatographic separation employed Poroshell 120 EC-C18 column (2.7 μm, 50 × 3.0 mm) with isocratic elution (20% acetonitrile with 0.1% formic acid) and 5-minute run time. Positive electrospray ionization with multiple reaction monitoring transitions (m/z 192.1 → 146.1 for 5-HIAA; 197.1 → 151.1 for 5-HIAA-d
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