Evidence map›Paper›PMID 42604867›Full record

ArticleAnalytical and bioanalytical chemistry2026

A label-free and highly sensitive fluorescence aptasensor for enrofloxacin detection based on G-triplex-mediated signal amplification.

Junli Feng, Zijun Wang, Peiqin Mao, Jingjing Liang, Xiaohui Wan, Yuqian Shen, Yunkai Wu, Kang Zhong, Like Gong, Xiaozheng Liu and 1 more

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Junli FengZhejiang Key Laboratory of Food Microbiology and Nutritional Health, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, 310018, China.
Zijun WangZhejiang Key Laboratory of Food Microbiology and Nutritional Health, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, 310018, China.
Peiqin MaoZhejiang Provincial Institute for Food and Drug Control, Hangzhou, 310052, China.
Jingjing LiangZhejiang Provincial Institute for Food and Drug Control, Hangzhou, 310052, China.
Xiaohui WanChangshan Inspection and Testing Institute, Quzhou, 324200, China.
Yuqian ShenChangshan Inspection and Testing Institute, Quzhou, 324200, China.
Yunkai WuKey Laboratory for Traceability and Efficacy Research of Genuine Medicinal Materials, Hangzhou Linping Hospital of Traditional Chinese Medicine, Linping, 311106, China.
Kang ZhongThe Women's and Children's Hospital of Linping District, Linping, Hangzhou, 311199, China.
Like GongHangzhou Center for Disease Control and Prevention (Hangzhou Health Supervision Institution), Hangzhou, 310021, China. lskane@163.com.
Xiaozheng LiuChangshan Inspection and Testing Institute, Quzhou, 324200, China. liu-xiao-zheng@163.com.
Qing ShenZhejiang Key Laboratory of Food Microbiology and Nutritional Health, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, 310018, China. leonqshen@163.com.

Funding

Linping District Medical and Health Science and Technology Project LPWJ2023-02-13Linping District Medical and Health Science and Technology Project LPWJ2024-02-672the "Pioneer" and "Leading Goose" Research and Development Program of Zhejiang 2025C02126the State Administration for Market Regulation Science and Technology Project 2023MK057the Zhejiang Province Traditional Chinese Medicine Science and Technology Plan 2026ZL0700
6 · The paper itself

Abstract

Enrofloxacin (ENR), a third-generation fluoroquinolone veterinary antibiotic, is widely used in aquaculture and animal husbandry for bacterial infection control due to its broad-spectrum antimicrobial activity, potent bactericidal efficacy, and cost-effectiveness. However, its excessive and non-standard use has caused widespread residues in animal-derived foods, which pose severe risks to human gastrointestinal, nervous and hepatorenal systems, induce bacterial resistance, and threaten global food safety and ecological sustainability. Herein, we developed a label-free, rapid, and highly sensitive fluorescence aptasensor for ENR detection based on G-triplex signal amplification. The ENR-specific aptamer was pre-hybridized with a guanine-rich sequence to suppress G-triplex formation and maintain a low thioflavin T (ThT) background. Upon ENR addition, target binding releases the guanine-rich strand, which folds into a stable G-triplex and strongly enhances ThT fluorescence for quantitative detection. Under optimal conditions, this aptasensor showed a linear range of 1.0 nM to 1.0 μM (R

Indexed as

AptamerEnrofloxacinFluorescence detectionFood safetyG-TriplexThioflavin T

Identifiers

PMID42604867

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.