Evidence map›Paper›PMID 42604813›Full record

ArticleEnvironmental microbiology2026

Genome-Guided Dissection of ser and shlB Uncovers Multifactorial Virulence Mechanisms of Serratia marcescens PXG6 in Plutella xylostella.

Muhammad Rehan Akhtar, Jun Ma, Yan Sun, Xiaofeng Xia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Muhammad Rehan AkhtarState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.ORCID https://orcid.org/0009-0001-9647-1530
Jun MaState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.ORCID https://orcid.org/0009-0000-1358-8382
Yan SunState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.ORCID https://orcid.org/0009-0000-5450-9687
Xiaofeng XiaState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.ORCID https://orcid.org/0000-0001-9522-7459

Funding

Science and Technology Innovation Fund of Fujian Agriculture and Forestry University KFB23024A
6 · The paper itself

Abstract

The rapid evolution of pesticide resistance in Plutella xylostella has reduced the effectiveness of conventional pest control methods in cruciferous crops. This study investigated the virulence of Serratia marcescens PXG6 against P. xylostella and identified two putative virulence factors, ser (serralysin) and shlB (hemolysin transporter). Whole-genome sequencing revealed a GC content of 59.65%, 4650 predicted genes, multiple secretion systems (types I, V, and VI), and 715 putative virulence-related genes. Bioinformatic analyses predicted type IV pili, serralysin, hemolysin, and flagella as pathogenic determinants. Purified prodigiosin exhibited concentration-dependent insecticidal activity, whereas bacterial proteins alone caused little larval mortality, indicating that full virulence depends on live bacterial cells. Gene knockout mutants Δser and ΔshlB exhibited altered growth, motility, biofilm formation, and stress tolerance. Deletion of shlB significantly reduced hemolytic activity, protein secretion, and virulence against P. xylostella, whereas Δser had minimal impact on larval survival. Overall, PXG6 pathogenicity involves multiple bacterial components, with shlB playing a key role in virulence. These findings improve our understanding of the pathogenic mechanisms of PXG6 and support the development of S. marcescens-based biocontrol strategies against P. xylostella.

Indexed as

Bacterial ProteinsHemolysin ProteinsMothsSerratia marcescensVirulence FactorsAnimalsGenome, BacterialLarvaVirulenceBacterial ProteinsHemolysin ProteinsVirulence Factorsbiocontrol strategiesHemolysin transporter (shlB)Plutella xylostellaSerralysin (ser)Serratia marcescens PXG6

Identifiers

PMID42604813
PMCPMC13477846

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.