Evidence map›Paper›PMID 42603194›Full record

ArticleBiodegradation2026

Biosurfactant-nonionic mixed micelles enhance heavy crude oil bioremediation: interfacial synergy and multilevel microbial responses.

Yang Wang, Ziyue Shang, Fei Wu, Yuhua Su, Liangzhen Du, Hailiang Yin, Qiyou Liu

Abstract read
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Article in Biodegradation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yang WangCollege of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, PR China.
Ziyue ShangCollege of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, PR China.
Fei WuNorthern Region Persistent Organic Pollutant Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, St. John's, NL, A1B 3X5, Canada.
Yuhua SuCollege of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, PR China.
Liangzhen DuCPECC EastChina Environmental and Geotechnical Engineering Branch, Qingdao, 266071, China.
Hailiang YinDongying Oildom Pollution Control Engineering Technology Key Laboratory, Dongying, 257061, China.
Qiyou LiuCollege of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, PR China. liuqiyou@upc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To address the water-oil-cell interfacial mass-transfer barrier limiting microbial degradation of heavy crude oil, this study evaluated a biosurfactant-nonionic mixed-micelle strategy that integrates interfacial performance with microbial compatibility. Four binary surfactant systems were screened using the petroleum hydrocarbon-degrading consortium B10. Among them, the rhamnolipid/Triton X-100 formulation, designated RT, showed the best overall balance of mixed-micelle synergy, crude-oil solubilization, microbial growth compatibility, and apparent hydrocarbon attenuation. After 7 d, RT achieved an apparent attenuation efficiency of 60.0%, compared with 21.9% in the surfactant-free B10 control, with the largest increases observed for the C16-C22 and C23-C32 fractions. RT treatment was accompanied by increased protein-like extracellular polymeric substance (EPS) content, changes in FTIR and fluorescence characteristics, a more negative zeta potential, and greater apparent cell-surface hydrophobicity. Metatranscriptomic analysis further revealed higher transcript abundance of genes associated with motility and chemotaxis, membrane transport, fatty acid metabolism, and the tricarboxylic acid cycle. Together, these multidimensional observations support a proposed mechanism model in which mixed-micelle interfacial regulation is associated with EPS compositional responses, cell-surface property changes, and consortium-level transcriptional adjustments, rather than solubilization alone.

Indexed as

BacteriaBiosurfactantsMicellesPetroleumSurface-Active AgentsBiodegradation, EnvironmentalGlycolipidsHydrocarbonsGlycolipidsHydrocarbonsMicellesPetroleumrhamnolipidSurface-Active AgentsCell-surface propertiesEPS compositional responsesHeavy crude oilHydrocarbon attenuationInterfacial regulationMixed surfactants

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.