Evidence map›Paper›PMID 42603138›Full record

ArticleMicrobiology spectrum2026

Analytical performance of a multi-target open real-time PCR assay for simultaneous detection of tuberculosis, non-tuberculous mycobacteria, and drug resistance in a high-burden setting.

Zeeshan Sidiq, Ankita Anand, Payal Tyagi, Kaushal Kumar Dwivedi, Sanjay Rajpal, Kamal Kishore Chopra

Abstract readEvaluation Study
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zeeshan SidiqNew Delhi Tuberculosis Centre, New Delhi, Delhi, India.ORCID 0000-0003-1845-9912
Ankita AnandNew Delhi Tuberculosis Centre, New Delhi, Delhi, India.
Payal TyagiNew Delhi Tuberculosis Centre, New Delhi, Delhi, India.
Kaushal Kumar DwivediNew Delhi Tuberculosis Centre, New Delhi, Delhi, India.
Sanjay RajpalNew Delhi Tuberculosis Centre, New Delhi, Delhi, India.
Kamal Kishore ChopraNew Delhi Tuberculosis Centre, New Delhi, Delhi, India.ORCID 0000-0003-3306-5129

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Timely diagnosis of tuberculosis (TB) and drug resistance is crucial for effective disease control. Multiplex open molecular platforms capable of simultaneously detecting IMPORTANCE: Despite major advances in molecular diagnostics, critical gaps persist in tuberculosis (TB) detection, particularly the limited identification of isoniazid resistance and inadequate differentiation from non-tuberculous mycobacteria. In this study, a multiplex open real-time PCR assay demonstrated high analytical accuracy for simultaneous detection of

Indexed as

Drug Resistance, BacterialMycobacterium Infections, NontuberculousMycobacterium tuberculosisNontuberculous MycobacteriaReal-Time Polymerase Chain ReactionTuberculosisAntitubercular AgentsHumansMicrobial Sensitivity TestsMultiplex Polymerase Chain ReactionRifampinSensitivity and SpecificityAntitubercular AgentsRifampinisoniazid resistancemolecular diagnosticsmultiplex real-time PCRnon-tuberculous mycobacteriarifampicin resistancetuberculosis

Identifiers

PMID42603138
PMCPMC13689452

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.