Evidence map›Paper›PMID 42603026›Full record

ArticleCureus2026

Comparative Evaluation of Conventional and Molecular Methods for the Diagnosis of Onychomycosis.

Aditi Dey, Shikha Paul, Sadia Afroz, Najmun Nahar Dina, Shuvro Roy, Shatabdi Mallick, Moshabha Akter Anika, Samah Binte Latif, Raisa Enayet Badhan

Abstract read
In one paragraph

Article in Cureus, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Aditi DeyMicrobiology, Community Based Medical College Bangladesh, Dhaka, BGD.
Shikha PaulMicrobiology, Sir Salimullah Medical College, Dhaka, BGD.
Sadia AfrozLaboratory Medicine, National Institute of Traumatology and Orthopaedic Rehabilitation, Dhaka, BGD.
Najmun Nahar DinaMicrobiology, Sir Salimullah Medical College, Dhaka, BGD.
Shuvro RoyPaediatrics, 250 Bed General Hospital, Brahmanbaria, Brahmanbaria, BGD.
Shatabdi MallickMicrobiology, Khulna Medical College, Khulna, BGD.
Moshabha Akter AnikaMicrobiology, Directorate General of Health Services, Dhaka, BGD.
Samah Binte LatifMicrobiology, Dhaka Community Medical College, Dhaka, BGD.
Raisa Enayet BadhanMicrobiology, National Institute of Burn and Plastic Surgery, Dhaka, BGD.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background Onychomycosis is a common fungal infection of the nail that often presents diagnostic challenges. Conventional laboratory methods such as microscopy and culture may show limited sensitivity, leading to delayed diagnosis and treatment. Molecular methods such as polymerase chain reaction (PCR) may improve diagnostic accuracy. Objectives To compare different diagnostic methods for onychomycosis by evaluating their sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). Methods This cross-sectional descriptive study was conducted from July 2023 to June 2024 at Sir Salimullah Medical College (SSMC), Dhaka. A total of 139 nail samples were collected from patients with clinically suspected onychomycosis attending the outpatient department of Dermatology and Venereology. The samples were examined by potassium hydroxide (KOH) mount microscopy, fungal culture, acridine orange stain fluorescence microscopy, periodic acid-Schiff (PAS) stain, and PCR. Laboratory investigations were conducted in the Department of Microbiology at SSMC and at Bangladesh Medical University (BMU). Culture was considered the gold standard, and the diagnostic performances of other methods were evaluated. Results Among the 139 samples, KOH mount microscopy detected 65 (46.79%) cases, PAS stain detected 31 (22.30%) cases, acridine orange fluorescence microscopy detected 68 (48.92%) cases, and PCR detected 98 (70.50%) cases. Dermatophytes were isolated in 70 (50.36%) cases and

Indexed as

acridine orange fluorescence microscopyfungal culturekoh microscopyonychomycosispas stainpcr

Identifiers

PMID42603026
PMCPMC13475716

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.