Evidence map›Paper›PMID 42602612›Full record

ArticlePeerJ2026

Simultaneous detection of thalassemia, hemoglobinopathies, and G6PD variants using long-read nanopore sequencing: genetic complexity and heterogeneity in Thailand.

Punchita Rujirachaivej, Praew Phermthong, Paweesuda Rattanakoch, Lanlana Treeravatananon, Narisara Saelee, Supasiri Chaweewattanaskul, Suthavee Boonlikit, Arisa Koyvanich, Sitthichai Panyasai, Rossarin Karnpean and 1 more

Abstract read
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In one paragraph

Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Punchita RujirachaivejDepartment of Pathology, Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Praew PhermthongMedical Genetic and Genomics Laboratory Unit, Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Paweesuda RattanakochMedical Genetic and Genomics Laboratory Unit, Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Lanlana TreeravatananonFaculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Narisara SaeleeFaculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Supasiri ChaweewattanaskulFaculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Suthavee BoonlikitFaculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Arisa KoyvanichFaculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Sitthichai PanyasaiDepartment of Medical Technology, School of Allied Health Sciences, University of Phayao, Phayao, Thailand.
Rossarin KarnpeanDepartment of Pathology, Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.
Wittaya JomouiDepartment of Pathology, Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Ongkharak, Nakhon Nayok, Thailand.ORCID 0000-0002-6035-3006

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thalassemia, hemoglobinopathies, and glucose-6-phosphate dehydrogenase (G6PD) deficiency represent highly prevalent and genetically heterogeneous inherited hematologic disorders or diseases in Thailand. Conventional molecular approaches require multiple targeted assays to detect deletional and non-deletional variants across globin gene clusters and the G6PD gene, resulting in increased complexity, turnaround time, and cost. We developed and analytically validated a comprehensive long-read sequencing workflow using Oxford Nanopore Technologies for simultaneous detection of pathogenic variants in

Indexed as

Glucosephosphate DehydrogenaseGlucosephosphate Dehydrogenase DeficiencyHemoglobinopathiesNanopore SequencingThalassemiaGenotypeHumansMutationThailandGlucosephosphate DehydrogenaseDiseasesG6PDHemoglobinopathiesLong-read sequencingNanoporeThalassemia

Identifiers

PMID42602612
PMCPMC13475388

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.