In silico and In vitro studies of EGFR kinase and Anilide derivatives as inhibitors against Breast Cancer.

Mala M Parab, Pramodkumar P Gupta, Shraddha Shetty, Santosh S Chhajed, Geetanjali Shukla, Kshitij Bapat, Debjani Dasgupta, Prerona Boruah

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In one paragraph

Article in Biotechnology notes (Amsterdam, Netherlands), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Mala M ParabSchool of Biotechnology and Bioinformatics, D Y Patil Deemed to Be University, Navi Mumbai, 400614, Maharashtra, India.

Pramodkumar P GuptaSchool of Biotechnology and Bioinformatics, D Y Patil Deemed to Be University, Navi Mumbai, 400614, Maharashtra, India.

Shraddha ShettySchool of Biotechnology and Bioinformatics, D Y Patil Deemed to Be University, Navi Mumbai, 400614, Maharashtra, India.

Santosh S ChhajedMET Institute of Pharmacy, MET, Nashik, Maharashtra, India.

Geetanjali ShuklaSchool of Biotechnology and Bioinformatics, D Y Patil Deemed to Be University, Navi Mumbai, 400614, Maharashtra, India.

Kshitij BapatSchool of Biotechnology and Bioinformatics, D Y Patil Deemed to Be University, Navi Mumbai, 400614, Maharashtra, India.

Debjani DasguptaSchool of Biotechnology and Bioinformatics, D Y Patil Deemed to Be University, Navi Mumbai, 400614, Maharashtra, India.

Prerona BoruahSchool of Biotechnology and Bioinformatics, D Y Patil Deemed to Be University, Navi Mumbai, 400614, Maharashtra, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The benzanilide scaffold is well known for its biological activities, such as antifungal, antibacterial, herbicide, and anti-tumor activity. Benzanilides with halogen substitution were computationally designed and screened against EGFR kinase, with erlotinib as a reference compound, using molecular docking. Two compounds were synthesized by the Schotten-Baumann reaction and confirmed based on FT-IR spectrophotometry, TLC, and sharp, narrow-range melting point analyses. The synthesized compounds were tested for antiproliferative activity and acute toxicity using the MTT assay with the (MCF-7) human breast cancer cell line. The effect of the compounds on non-cancerous cells in the human body was tested using blood lymphocytes. The mechanism of action evaluated by an in vitro cytotoxicity study revealed IC

Indexed as

BenzanilidesChemotherapeuticEGFR-TKIC50MCF-7MTT assaySchotten-Baumann

Identifiers

PMID42602599

DOI10.1016/j.biotno.2026.06.002

PMCPMC13474251

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.