Ascorbic acid mitigates oxidative structural degradation in bovine spermatozoa: a label-free quantitative phase microscopy study.

Harpreet Kaur, Shubham Tiwari, Aman Kumar, Sandeep Kumar Jha, Satish Kumar Dubey, Dalip Singh Mehta

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Article in Veterinary and animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Harpreet KaurCenter for Sensors, Instrumentation and Cyber Physical System Engineering (SeNSE), Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.

Shubham TiwariCenter for Sensors, Instrumentation and Cyber Physical System Engineering (SeNSE), Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.

Aman KumarLala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, Haryana, 125004, India.

Sandeep Kumar JhaCenter for Biomedical Engineering (CBME), Indian Institute of Technology Delhi, Hauz-Khas, New Delhi 110016, India.

Satish Kumar DubeyCenter for Sensors, Instrumentation and Cyber Physical System Engineering (SeNSE), Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.

Dalip Singh MehtaCenter for Sensors, Instrumentation and Cyber Physical System Engineering (SeNSE), Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxidative stress is a key factor in low fertility outcomes during assisted reproduction technology (ART) and contributes to poor sperm quality. Conventional sperm assessment relies on bright-field microscopy, which lacks the quantitative sensitivity to resolve subcellular structural and biophysical changes without exogenous contrast agents, a requirement that can introduce cytotoxic effects and compromise cell viability. This study uses quantitative phase microscopy (QPM) as a label-free, high-throughput method to demonstrate how ascorbic acid (Vitamin C) reduces the impact of oxidative structural degradation in Sahiwal bovine spermatozoa. To illustrate this pathology, severe oxidative stress was experimentally induced using hydrogen peroxide (H

Indexed as

Ascorbic acidAssisted reproductive technologiesBovine spermatozoaLabel-freeOxidation stressQuantitative phase microscopy

Identifiers

PMID42602564

DOI10.1016/j.vas.2026.100800

PMCPMC13475238

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