ArticleFrontiers in insect science2026
Molecular monitoring and multiplex qPCR-based detection of
Article in Frontiers in insect science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Apple production in Kazakhstan is highly vulnerable to damage caused by fruit-infesting tortricid pests, particularly codling moths (Lepidoptera: Tortricidae). Accurate and rapid species identification is essential for effective pest management and phytosanitary monitoring in apple orchards. Methods: Field surveys were conducted in apple orchards of southern and southeastern Kazakhstan to investigate the codling moth complex. Collected specimens were identified morphologically and confirmed by sequencing a mitochondrial cytochrome c oxidase subunit I (COI) region. Comparative COI sequence analysis was used to develop a multiplex quantitative polymerase chain reaction assay employing Minor Groove Binder hydrolysis probes for simultaneous detection and differentiation of Results: Field surveys identified Discussion: The developed COI-based multiplex quantitative polymerase chain reaction assay provides a rapid, sensitive, and reliable tool for simultaneous identification of
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