Evidence map›Paper›PMID 42599797›Full record

ArticleCell reports2026

EWSR1::FLI1 promotes neocentromere formation in ewing sarcoma cells.

Risa Kitagawa, Ediri E Metitiri, Bin Yu, Changxian Shen, Alexander J R Bishop, Siyuan Zheng, Peter J Houghton, Katsumi Kitagawa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Risa KitagawaGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Molecular Medicine, University of Texas Health at San Antonio, San Antonio, TX 78229, USA.
Ediri E MetitiriGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Molecular Medicine, University of Texas Health at San Antonio, San Antonio, TX 78229, USA.
Bin YuGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Molecular Medicine, University of Texas Health at San Antonio, San Antonio, TX 78229, USA.
Changxian ShenGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Molecular Medicine, University of Texas Health at San Antonio, San Antonio, TX 78229, USA.
Alexander J R BishopGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Cell Systems and Anatomy, University of Texas Health at San Antonio, San Antonio, TX 78229, USA; Center for Childhood Cancer Research, Abigail Wexner Research, Institute at Nationwide Children's Hospital, Research Building IV, 575 Children's Crossroad, Columbus, OH 43215, USA.
Siyuan ZhengGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Population Health Sciences, University of Texas Health at San Antonio, San Antonio, TX 78229, USA.
Peter J HoughtonGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Molecular Medicine, University of Texas Health at San Antonio, San Antonio, TX 78229, USA.
Katsumi KitagawaGreehey Children's Cancer Research Institute, Mays Cancer Center, University of Texas Health at San Antonio, 8403 Floyd Curl Drive, San Antonio, TX 78229, USA; Department of Molecular Medicine, University of Texas Health at San Antonio, San Antonio, TX 78229, USA. Electronic address: kitagawak@uthscsa.edu.

Funding

THE ROLE OF EWSRI AT CENTROMERE-R01GM147636 · NIGMS · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI KATSUMI KITAGAWA · 2023 to 2026
$1.5M
The role of CENP-A in the response to DNA double-strand breaksR21ES033354 · NIEHS · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI KITAGAWA, KATSUMI · 2022 to 2023
$426k
NIEHS NIH HHS R21 ES033354NIGMS NIH HHS R01 GM147636
6 · The paper itself

Abstract

Ewing sarcoma (EwS) is characterized by chimeric fusions such as EWSR1::FLI1. Chromosome gain or loss is prevalent in EwS, and this chromosome instability (CIN) phenotype correlates with poor treatment outcomes. We previously showed that CENP-A, a centromere-specific histone H3 variant, interacts with EWSR1 via its SYGQ2 motif, an interaction extending to the EWSR1::FLI1 fusion protein. CENP-A is highly expressed in EwS cells, driven by EWSR1::FLI1. EwS cells exhibit significant CIN, mitigated by depleting EWSR1::FLI1 or CENP-A, and frequently assemble neocentromeres. Using CENP-A ChIP-seq, we identify neocentromere-associated DNA sequences, and ChIP assays confirm EWSR1::FLI1 binds these sequences, suggesting EWSR1::FLI1 recruits CENP-A to drive neocentromere assembly. Notably, overexpressing EWSR1::FLI1, but not CENP-A, induces neocentromeres in non-EwS cells. These neocentromeres likely produce dicentric chromosomes, explaining the high incidence of chromosome bridges and chromoplexy underlying the CIN phenotype in EwS.

Indexed as

CENP-Acentromerechromoplexychromosome instabilityCP: cell biologydicentric chromosomeEwing sarcomaEWSR1(EWS)EWSR1::FLI1kinetochoreneocentromere

Identifiers

PMID42599797
PMCPMC13580748

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.