Purification, identification, and characterization of a novel bioactive peptide LP-15 with antimicrobial and antioxidant activities produced by Lactobacillus pentosus BL-15 isolated from Asterias.

Wenjuan Zhu, Qibin Li, Huifang Tang, Abazar Ghorbani, Ying Liu

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Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Wenjuan ZhuCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, China.

Qibin LiCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, China.

Huifang TangCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, China.

Abazar GhorbaniState Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing, 210037, China.

Ying LiuCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, China. liuying@gdou.edu.cn.

Funding

Guangdong Province Science and Technology Project 2017A030303079

6 · The paper itself

Abstract

Bioactive peptides from lactic acid bacteria (LAB) are gaining attention as natural antimicrobial and antioxidant agents for food applications. In this study, a novel bioactive peptide, LP-15, derived from Lactobacillus pentosus BL-15 isolated from Asterias collected on Naozhou Island, China, was characterized. The cell-free supernatant of strain BL-15 displayed antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Listeria monocytogenes) and Gram-negative bacteria (Escherichia coli and Vibrio parahaemolyticus), as well as antioxidant activities including DPPH, hydroxyl radical scavenging, ABTS radical scavenging, and total reducing capacity assays. Based on these results, strain BL-15 was selected for peptide purification. Peptide LP-15 was purified using ethyl acetate (EtOAc) extraction, SP-Sepharose Fast Flow cation exchange chromatography, Sephadex LH-20 gel chromatography, and reversed-phase high-performance liquid chromatography (RP-HPLC), which exhibited antimicrobial activity against L. monocytogenes and notable antioxidant activity (DPPH radical scavenging rate: 78.72%). Mass spectrometry identified LP-15 as an 11-amino-acid peptide (LEVQAVSKNKL) with a molecular weight of 1279.66 Da, showing ~ 90% sequence similarity to the protein G2/mitotic-specific cyclin-B. LP-15 retained antimicrobial and antioxidant activities after heat and UV treatments and remained stable over a pH range of 2 ~ 6. These findings highlight LP-15 as a promising natural preservative candidate for food applications.

Indexed as

Anti-Bacterial AgentsAntimicrobial PeptidesAntioxidantsLactiplantibacillus pentosusPeptidesChinaChromatography, High Pressure LiquidGram-Negative BacteriaGram-Positive BacteriaMicrobial Sensitivity TestsAnti-Bacterial AgentsAntimicrobial PeptidesAntioxidantsPeptidesLactobacillus pentosusNatural preservativePeptide characterizationPurificationRadical scavenging activity

Identifiers

PMID42599602

DOI10.1007/s11274-026-05196-2

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.