ArticlePhysiologia plantarum
EMB2217 Is a Mitochondrial PPR-SMR Protein Required for the Splicing of Multiple Group II Introns During Arabidopsis Development.
Article in Physiologia plantarum. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
Pentatricopeptide repeat (PPR) proteins are key regulators of the organellar RNA metabolism in plants. However, the functions of mitochondrial PPR proteins belonging to the subclass of P-type PPR factors containing the SMR domain remain much less understood. Here, we characterize the EMBRYO DEFECTIVE 2217 (EMB2217/At1g79490), an essential PPR-SMR factor in Arabidopsis thaliana. T-DNA insertional lines at the AT1G79490 gene-locus exhibit embryonic arrest at the late heart stage and display defective germination and seedling establishment. Partial complementation using an ABI3 promoter-driven strategy enables efficient germination and the rescue of homozygous emb2217 plantlets. The pABI3::EMB2217 emb2217 -/- seedlings display severe growth defects due to impaired mitochondrial function, tightly associated with impaired OXPHOS activity. Analyses of mitochondrial RNA profiles reveal that EMB2217 is required for the processing of multiple group II introns that reside in the coding regions of several complex I (CI) subunits, the cox2 subunit of CIV, and the ribosomal rps3 factors. Our data further show that RNA maturation defects induce alternative electron transport and stress-response pathways, which are associated with developmental defects and modulation of photosynthetic and cellular metabolic processes. Together, we identify EMB2217 as a general mitochondrial splicing factor whose loss compromises OXPHOS biogenesis and function, cellular energy supply, and plant development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.