Evidence map›Paper›PMID 42595755›Full record

ArticleNature communications2026

Genome-wide CRISPR screening identifies cellular factors controlling nonviral genome editing efficiency.

Shivani Saxena, Meha Kabra, Amr A Abdeen, Diana M Tabima, Divya Sinha, Piper A Rawding, Min Zhu, Ruosen Xie, Tanvi Kulkarni, Giovanni M Hanstad and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Shivani Saxena *Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0009-0002-0012-1673
Meha Kabra *McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA.
Amr A AbdeenDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0003-4071-6620
Diana M TabimaDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Divya SinhaMcPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-5854-0056
Piper A RawdingWisconsin Institute of Discovery, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-9233-9700
Min ZhuWisconsin Institute of Discovery, University of Wisconsin-Madison, Madison, WI, USA.
Ruosen XieWisconsin Institute of Discovery, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-0249-0002
Tanvi KulkarniDepartment of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA.
Giovanni M HanstadWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Maria A Fernandez ZepedaWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
David M GammMcPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-8152-613X
Bikash R PattnaikMcPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-5385-358X
Shaoqin GongWisconsin Institute of Discovery, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0001-9447-2938
Krishanu SahaDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. ksaha@wisc.edu.ORCID http://orcid.org/0000-0003-2837-0858

Funding

The CRISPR Vision Program: Nonviral Genome Editing Platforms to Treat Inherited Retinal Channelopathies Epqt SupplementU19NS132296 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI Krishanu Saha · 2023 to 2026
$36.8M
Assembly of Novel Gene Editing Particles to Understand Genome Surgery in Patient-Derived CellsR35GM119644 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SAHA, KRISHANU · 2016 to 2025
$4.0M
NIGMS NIH HHS R35 GM119644NINDS NIH HHS U19 NS132296U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM119644U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) U19NS132296
6 · The paper itself

Abstract

To systematically map cellular factors constraining nonviral genome editing, influencing uptake and intracellular trafficking, we develop a genome-wide CRISPR screening platform linking perturbation of 19,114 genes to editing outcomes in human cells. We identify six negative regulators of delivery whose depletion increases editing efficiency by up to six-fold across diverse payloads, loci, and cell types. We test the top two factors, GJB2 and BET1L, in two distinct human models: correction of a pathogenic adenine base mutation in KCNJ13 and introduction of a cytosine base mutation in the GABA

Indexed as

CRISPR-Cas SystemsGene EditingClustered Regularly Interspaced Short Palindromic RepeatsConnexin 26ConnexinsGenome, HumanHEK293 CellsHumansMutationPotassium Channels, Inwardly RectifyingReceptors, GABA-AConnexin 26ConnexinsGJB2 protein, humanPotassium Channels, Inwardly RectifyingReceptors, GABA-A

Identifiers

PMID42595755
PMCPMC13473100

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.